An efficient transient expression system for gene function analysis in rose.
Identifieur interne : 000254 ( Main/Exploration ); précédent : 000253; suivant : 000255An efficient transient expression system for gene function analysis in rose.
Auteurs : Jun Lu [République populaire de Chine] ; Mengjuan Bai [République populaire de Chine] ; Haoran Ren [République populaire de Chine] ; Jinyi Liu [République populaire de Chine] ; Changquan Wang [République populaire de Chine]Source :
- Plant methods [ 1746-4811 ] ; 2017.
Abstract
Background
Roses are widely used as garden ornamental plants and cut flowers.
Results
A convenient and highly efficient
Conclusions
This transient transformation system was validated as a robust and efficient platform, thus providing a new option for gene function and signaling pathway investigation in roses and further extending the utility of
DOI: 10.1186/s13007-017-0268-1
PubMed: 29299050
PubMed Central: PMC5740963
Affiliations:
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city</wicri:noRegion></affiliation></author><author><name sortKey="Bai, Mengjuan" sort="Bai, Mengjuan" uniqKey="Bai M" first="Mengjuan" last="Bai">Mengjuan Bai</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</nlm:affiliation><wicri:noCountry code="subField">210095 Jiangsu Province People's Republic of China</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city</wicri:regionArea><wicri:noRegion>Nanjing city</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Haoran" sort="Ren, Haoran" uniqKey="Ren H" first="Haoran" last="Ren">Haoran Ren</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</nlm:affiliation><wicri:noCountry code="subField">210095 Jiangsu Province People's Republic of China</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city</wicri:regionArea><wicri:noRegion>Nanjing city</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Jinyi" sort="Liu, Jinyi" uniqKey="Liu J" first="Jinyi" last="Liu">Jinyi Liu</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</nlm:affiliation><wicri:noCountry code="subField">210095 Jiangsu Province People's Republic of China</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city</wicri:regionArea><wicri:noRegion>Nanjing city</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Changquan" sort="Wang, Changquan" uniqKey="Wang C" first="Changquan" last="Wang">Changquan Wang</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</nlm:affiliation><wicri:noCountry code="subField">210095 Jiangsu Province People's Republic of China</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city</wicri:regionArea><wicri:noRegion>Nanjing city</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:29299050</idno><idno type="pmid">29299050</idno><idno type="doi">10.1186/s13007-017-0268-1</idno><idno type="pmc">PMC5740963</idno><idno type="wicri:Area/Main/Corpus">000197</idno><idno type="wicri:explorRef" wicri:stream="Main" 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China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city</wicri:regionArea><wicri:noRegion>Nanjing city</wicri:noRegion></affiliation></author><author><name sortKey="Bai, Mengjuan" sort="Bai, Mengjuan" uniqKey="Bai M" first="Mengjuan" last="Bai">Mengjuan Bai</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</nlm:affiliation><wicri:noCountry code="subField">210095 Jiangsu Province People's Republic of China</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city</wicri:regionArea><wicri:noRegion>Nanjing city</wicri:noRegion></affiliation></author><author><name sortKey="Ren, Haoran" sort="Ren, Haoran" uniqKey="Ren H" first="Haoran" last="Ren">Haoran Ren</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</nlm:affiliation><wicri:noCountry code="subField">210095 Jiangsu Province People's Republic of China</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city</wicri:regionArea><wicri:noRegion>Nanjing city</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Jinyi" sort="Liu, Jinyi" uniqKey="Liu J" first="Jinyi" last="Liu">Jinyi Liu</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</nlm:affiliation><wicri:noCountry code="subField">210095 Jiangsu Province People's Republic of China</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city</wicri:regionArea><wicri:noRegion>Nanjing city</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Changquan" sort="Wang, Changquan" uniqKey="Wang C" first="Changquan" last="Wang">Changquan Wang</name><affiliation><nlm:affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</nlm:affiliation><wicri:noCountry code="subField">210095 Jiangsu Province People's Republic of China</wicri:noCountry></affiliation><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city</wicri:regionArea><wicri:noRegion>Nanjing city</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant methods</title><idno type="ISSN">1746-4811</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>Background</b></p><p>Roses are widely used as garden ornamental plants and cut flowers. </p></div><div type="abstract" xml:lang="en"><p><b>Results</b></p><p>A convenient and highly efficient </p></div><div type="abstract" xml:lang="en"><p><b>Conclusions</b></p><p>This transient transformation system was validated as a robust and efficient platform, thus providing a new option for gene function and signaling pathway investigation in roses and further extending the utility of </p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29299050</PMID><DateRevised><Year>2020</Year><Month>10</Month><Day>01</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1746-4811</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Plant methods</Title><ISOAbbreviation>Plant Methods</ISOAbbreviation></Journal><ArticleTitle>An efficient transient expression system for gene function analysis in rose.</ArticleTitle><Pagination><MedlinePgn>116</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13007-017-0268-1</ELocationID><Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">Roses are widely used as garden ornamental plants and cut flowers. <i>Rosa chinensis</i> cv 'Old Blush' has been used as a model genotype in rose studies due to its contribution to recurrent flowering and tea scent traits of modern roses. The deficiency of efficient genetic transformation systems is a handicap limiting functional genetics studies of roses. <i>Agrobacterium</i>-mediated transient transformation offers a powerful tool for the characterization of gene function in plants.</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">A convenient and highly efficient <i>Agrobacterium</i> mediated genetic transformation protocol using <i>R. chinensis</i> cv 'Old Blush' seedlings in vitro as an expression system is described in this paper. The most important factor affecting transformation efficiency in this system is seedling age; 3/4-week-old rose shoots with or without roots from sub-culturing are optimal for transformation, requiring no complicated inoculation media, supplements, or carefully tuned plant growth conditions. This transient expression system was successfully applied to analysis of the gene promoter activities, DNA binding capacity of transcription factors, protein-protein interaction in physiological contexts using luciferase as a reporter gene.</AbstractText><AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">This transient transformation system was validated as a robust and efficient platform, thus providing a new option for gene function and signaling pathway investigation in roses and further extending the utility of <i>R. chinensis</i> cv 'Old Blush' as a model plant to study diverse gene function and signaling pathways in Rosaceae.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Jun</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bai</LastName><ForeName>Mengjuan</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ren</LastName><ForeName>Haoran</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Jinyi</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Changquan</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>College of Horticulture, Nanjing Agricultural University, No. 1 of Weigang, Nanjing City, 210095 Jiangsu Province People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>Key Laboratory of Landscaping of Ministry of Agriculture, Nanjing Agricultural University, Nanjing city, 210095 People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>12</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Methods</MedlineTA><NlmUniqueID>101245798</NlmUniqueID><ISSNLinking>1746-4811</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium</Keyword><Keyword MajorTopicYN="N">Roses</Keyword><Keyword MajorTopicYN="N">Shoots in vitro</Keyword><Keyword MajorTopicYN="N">Transient transformation</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>07</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2017</Year><Month>12</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>1</Month><Day>5</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29299050</ArticleId><ArticleId IdType="doi">10.1186/s13007-017-0268-1</ArticleId><ArticleId 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sort="Bai, Mengjuan" uniqKey="Bai M" first="Mengjuan" last="Bai">Mengjuan Bai</name><name sortKey="Liu, Jinyi" sort="Liu, Jinyi" uniqKey="Liu J" first="Jinyi" last="Liu">Jinyi Liu</name><name sortKey="Ren, Haoran" sort="Ren, Haoran" uniqKey="Ren H" first="Haoran" last="Ren">Haoran Ren</name><name sortKey="Wang, Changquan" sort="Wang, Changquan" uniqKey="Wang C" first="Changquan" last="Wang">Changquan Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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